QB-Ms2-2

QB-Ms2-2

Order

Species

Common Name(s)

Tobacco hornworm

Primary Designation

QB-Ms2-2

Accession No.

CVCL_0A17

Stage

Tissue

Initiator(s)

L. Jiang

References

Jiang, L., Li, G., Li, C., Granados, R. R., & Blissard, G. W. (2010). Growth characteristics and expression of recombinant proteins in three new cell lines from Manduca sexta (Lepidoptera: Sphingidae). Acta Entomologica Sinica, 53(11), 1227-1232.

Notes

Morphology: cells are generally spindle shaped; Growth characteristics: doubling time = 31 hr; Unique properties: SEAP High expression of SEAP type cell line, QB-M2-2, which may provide a source for the development of novel clones with higher production of recombinant proteins; this cell line showed higher level of secreted SEAP production in comparison with BTI-Tn5B1-4 cell line.

Medium

TNM-FH + 10% FBS

Status

Microorganism Propagation

Replicates Autographa californica multiple nucleohedrovirus (AcMNPV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu