RIRI-PX1
Order
Species
Common Name(s)
Asian swallowtail butterfly
Primary Designation
RIRI-PX1
Accession No.
CVCL_Z962
Stage
Tissue
Initiator(s)
X. Zhang
References
Zhang, X., Feng, Y., Ding, W. F., Chen, X. M., Wang, C. Y., & Ma, T. (2012). Characterization of a new insect cell line that is derived from the neonate larvae of Papilio xuthus (Lepidoptera: Papilionidae) and its susceptibility to AcNPV. Tissue and Cell, 44(3), 137-142.
Notes
Morphology: primarliy consists of spindle-shaped and spherical cells; size of the spindle-shaped cells are 10–38 µm × 5–10 µm, and the diameter range of the spherical cells is 10–13 µm; Growth characteristics: population-doubling times = 42.5 hr (P50) and 42.1 hr (P60); Karyology: heteroploidy of chromosomes is observed; average chromosome number ranged from 103 to 199 between passage 5 and passage 50; Authentication: identity confirmed by comparing the mitochondrial cytochrome c oxidase subunit I gene (COI) of RIRI-PX1 cells with P. xuthus eggs.
Medium
Grace's Insect medium + 20% FBS
Cell Culture Collection
Ying Feng, Research Institute of Resource Insects, Chinese Academy of Forestry, The Key Laboratory of Cultivating and Utilization of Resource Insects of State Forestry Administration, China.
yingf@hotmail.com
Status
Microorganism Propagation
Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
