Se301
Order
Species
Common Name(s)
Beet armyworm
Primary Designation
Se301
Accession No.
CVCL_Z520
Stage
Tissue
Initiator(s)
K. Hara
References
Hara, K., Funakoshi, M., & Kawarabata, T. (1995). A cloned cell line of Spodoptera exigua has a highly increased susceptibility to the Spodoptera exigua nuclear polyhedrosis virus. Canadian Journal of Microbiology, 41(12), 1111-1116; Yanase, T., Yasunaga, C., & Kawarabata, T. (1998). Replication of Spodoptera exigua nucleopolyhedrovirus in permissive and non-permissive lepidopteran cell lines. Acta Virologica, 42(5), 293-298; Beas-Catena, A., Sánchez-Mirón, A., García-Camacho, F., & Molina-Grima, E. (2011). Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV). Cytotechnology, 63(6), 543-552.
Notes
Origin: this is a clonal cell line derived from Se3FH; Unique properties: this line is very sensitive to the hydrodynamic shear rates in bioreactors but was able to adapt to suspension culture using the protective additives polyvinyl alcohol (PVA) or polyvinyl pyrrolidone (PVP) with the disaggregant dextran sulfate (DS).
Medium
TNM-FH + 10% FBS
Cell Culture Collection
Department of Virology, Wageningen University.
Status
Microorganism Propagation
Replicates Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV)
