Common Name(s): 
Cabbage moth
Primary Designation: 
Accession No.: 
H. Inoue
Inoue, H., & Mitsuhashi, J. (1985). Further establishment of continuous cell lines from larval fat bodies of the cabbage armyworm, Mamestra brassicae (Lepidoptera: Noctuidae). Applied Entomology and Zoology, 20(4), 496-498; Koike, M., & Sato, K. (1988). Culture of insect cell lines originated from Mamestra brassicae with autoclaved serum-free medium. In: Invertebrate and Fish Tissue Culture (Eds.: Kuroda Y., Kurstak E., Maramorosch K.). pp.7-8; Japan Sci. Soc., Tokyo; Inoue, H. (1989). Establishment of insect continuous cell lines and its utilization for virus multiplication in vitro. Japan Agricultural Research Quarterly, 23(1), 31-36; Mitsuhashi, J. (1989). Nutritional requirements of insect cells in vitro. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi, J.), pp. 3-20; CRC Press; Boca Raton; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Kawai, Y., & Mitsuhashi, J. (1997). An insect cell line discrimination method by RAPD-PCR. In Vitro Cellular & Developmental Biology-Animal, 33(7), 512-515.
Morphology: obtained cell line are all suspending cells and consists mostly of spherical cells in suspension, although various other shapes, especially elongated cells, also are seen; Growth characteristics: pupulation doubling time = 30 hr; Karyology: most metaphase plates cells contained more than 100 chromosomes.
Maramorosch's medium (MM) + 3% FBS
Microorganism Propagation: 
Chilo iridescent virus (CIV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV)