TN-368

TN-368

Order

Species

Common Name(s)

Cabbage looper

Primary Designation

TN-368

Accession No.

CVCL_C412

Stage

Tissue

Initiator(s)

W.F. Hink

References

Hink, W. F. (1970). Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, 226(5244), 466-467; McIntosh, A. H., & Rechtoris, C. (1974). Insect cells: colony formation and cloning in agar medium. In Vitro, 10(1-2), 1-5; Volkman, L. E., & Summers, M. D. (1975). Nuclear polyhedrosis virus detection: relative capabilities of clones developed from Trichoplusia ni ovarian cell line TN-368 to serve as indicactor cells in a plaque assay. Journal of Virology, 16(6), 1630-1637; Hink, W. F. (1976). Growth of the Trichoplusia ni (TN-368) cell line in suspension culture. In: Invertebrate Tissue Culture: Applications in Medicine, Biology and Agriculture (Eds: Kurstak, E., Maramorosch, K.), Academic Press, New York, pp. 297-300; Volkman, L. E., & Goldsmith, P. A. (1982). Generalized immunoassay for Autographa californica nuclear polyhedrosis virus infectivity in vitro. Applied and Environmental Microbiology, 44(1), 227-233; Koval, T. M. (1983). Intrinsic resistance to the lethal effects of X-irradiation in insect and arachanid cells. Proceedings of the National Academy of Sciences, 80(15), 4752-4755; Harvey, G. T., & Sohi, S. S. (1985). Isozyme characterization of 28 cell lines from five insect species. Canadian Journal of Zoology, 63(10), 2270-2276; Harvey, G. T., & Sohi, S. S. (1989). Isozyme characterization of 8 hymenopteran and 20 lepidopteran cell lines. In: Invertebrate Cell System Applications, Vol. I (Ed: Mitsuhashi, J.), CRC Press, Boca Raton, pp. 71-75; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Styer, S. C., & Griffiths, T. D. (1992). Effect of UVC light on growth, incorporation of thymidine, and DNA chain elongation in cells derived from the Indian meal moth and the cabbage looper. Radiation Research, 130(1), 72-78; Davis, T. R., Wickham, T. J., McKenna, K. A., Granados, R. R., Shuler, M. L., & Wood, H. A. (1993). Comparative recombinant protein production of eight insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 29(5), 388-390; Lin, C. L., Lee, J. C., Chen, S. S., Wood, H. A., Li, M. L., Li, C. F., and Chao, Y. C. (1999). Persistent HZ-1 infection in insect cells: evidence for insertion of viral DNA into host chromosomes and viral infection in a latent status. Journal of Virology, 73, 128-139; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Lynn, D (2007). Available lepidopteran insect cell lines. In: Methods In Molecular Biology, Vol. 338: Baculovirus and Insect Cell Expression Protocols (Ed: D. W. Murhammer), pp. 117-137, Humana Press Inc, Totowa, NJ.; Geisler, C., & Jarvis, D. L. (2018). Adventitious viruses in insect cell lines used for recombinant protein expression. Protein Expression and Purification, 144, 25-32; Bonning, B. C. (2019). The insect virome: opportunities and challenges. In: Insect Molecular Virology: Advances and Emerging Trends (Ed: Bonning, B. C), Caister Academic Press, Poole, UK, pp 1-12.

Notes

Growth characteristics: cells grow in suspension and tend to cluster in aggregates; population doubling time = 14.5 to 24 hr; Morphology: spindle-shaped cells: Karyology: typical of lepidopteran cells; Authentication: isozyme pattern analysis indicates unique patterns.

Medium

TNM-FH + 10% FBS

Status

Microorganism Propagation

Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV), Plutella xylostella mutiple nucleopolyhedrovirus (PxMNPV or PlxyMNPV), Trichoplusia ni single nucleopolyhedrovirus (TnSNPV), Galleria mellonella multiple nucleopolyhedrovirus (GmMNPV)

Adventitious Microorganism

Infected with the alphanodavirus TnCLV (Trichoplusi ni cell line virus) and, in some cases, Nudivirus (HzNV-1)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu