TN-CL1
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
TN-CL1
Accession No.
CVCL_Z257
Stage
Tissue
Initiator(s)
A. H. McIntosh
References
McIntosh, A. H., & Rechtoris, C. (1974). INSECT CELLS: COLONY FORMATION AND CLONING IN AGAR MEDIUM. In Vitro, 10(1-2), 1-5; McIntosh, A. H., & Ignoffo, C. M. (1989). REPLICATION OF AUTOGRAPHA CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS IN FIVE LEPIDOPTERAN CELL LINES. Journal of Invertebrate Pathology, 54(1), 97-102; McIntosh, A. H. (1991). IN VITRO INFECTIVITY OF A CLONAL ISOLATE OF SYNGRAPHA FALCIFERA (CELERY LOOPER) MULTIPLE NUCLEAR POLYHEDROSIS VIRUS. Journal of invertebrate pathology (Print), 57(3), 441-442; McIntosh, A. H., Barcenas, N., & Cate, J. R. (1992). REPLICATION OF AUTOGRAPHA CALIFORNICA BACULOVIRUS (ACMNPV) IN A COLEOPTERAN CELL LINE. In Vitro Cellular & Developmental Biology-Animal, 28(7-8), 557-559; McIntosh, A. H., Grasela, J. J., & Matted, R. L. (1996). IDENTIFICATION OF INSECT CELL LINES BY DNA AMPLIFICATION FINGERPRINTING (DAF). Insect Molecular Biology, 5(3), 187-195; Grasela, J. J., Mcintosh, A. H., Goodman, C. L., Wilson, L. E., & King, L. A. (2000). EXPRESSION OF THE GREEN FLUORESCENT PROTEIN CARRIED BY AUTOGRAPHA CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS IN INSECT CELL LINES. In Vitro Cellular & Developmental Biology-Animal, 36(3), 205-210; Alhag, A. K. N., Chao, Y. H., & Xin, P. J. (2007). IDENTIFICATION OF INSECT CELL LINES FROM 8 LEPIDOPTERAN SPECIES BY DNA AMPLIFICATION FINGERPRINTING. J. Appl. Sci, 7, 4040-4043.
Notes
Origin: clonal isolate of TN-368; Growth charcteristics: population doubling time ~5-days; Unique properties: able to produce AcMNPV.GFP, with the highest number of green fluorescent cells of those tested; Authentication: DNA amplification fingerprinting-polymerase chain reaction (DAF PCR) distinguishes this cell line from other lepidopteran insects.
Medium
EX-CELL 420 with or without 10% FBS
Cell Culture Collection
Cynthia Goodman, USDA, ARS, Biological Control of Insects Research Laboratory (BCIRL), Columbia, Missouri, USA.
Jacob.Corcoran@usda.gov
Status
Microorganism Propagation
Produces high levels of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) budded virus and moderate levels of occlusion bodies
