Tn-NVN

Tn-NVN

Order

Species

Common Name(s)

Cabbage looper

Primary Designation

Tn-NVN

Accession No.

CVCL_RT22

Stage

Tissue

Initiator(s)

A. Maghodia

References

Geisler, C., & Jarvis, D. L. (2018). ADVENTITIOUS VIRUSES IN INSECT CELL LINES USED FOR RECOMBINANT PROTEIN EXPRESSION. Protein Expression and Purification, 144, 25-32.

Notes

Origin: clonal isolate of TN-368; Unique properties: similar level of baculovirus-mediated recombinant protein production as parental cell line; Authentication: N-linked glycosylation pattern is similar to parental cell line; Growth characteristics: maximum cell density and doubling time is similar to parental cell line; Morphology: average cell diameter is similar to parental line; Microbial presence: mycoplasma-free; TnCLV-free (as determined by nested RT-PCR).

Medium

ESF-921

Cell Culture Collection

Dr. Don Jarvis, Department of Molecular Biology University of Wyoming, 1000 E. University Ave. Laramie, WY 82071.

Status

Adventitious Microorganism

Ribavirin-treatment effectively eliminated the alphanodavirus TnCLV (Trichoplusi ni cell line virus) from this clonal cell line

Patents

Maghodia, A., Geisler, C., & Jarvis, D. (2017). World Intellectual Property Organization, International Bureau, International Publication Number WO 2017/075627 Al; Maghodia, A., Geisler, C., & Jarvis, D. (2018). U.S. Patent Application No. US 2018 / 0355311 A1 YOU HAD THIS NUMBER 15/772,476 (please check into this)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu