Tni-FNL
Order
Species
Common Name(s)
Cabbage looper
Primary Designation
Tni-FNL
Accession No.
CVCL_RY32
Stage
Tissue
Initiator(s)
J. L. Mehalko
References
Mehalko, J. L., & Esposito, D. (2016). ENGINEERING THE TRANSPOSITION-BASED BACULOVIRUS EXPRESSION VECTOR SYSTEM FOR HIGHER EFFICIENCY PROTEIN PRODUCTION FROM INSECT CELLS. Journal of Biotechnology, 238, 1-8.
Notes
Origin: derived from BTI-Tn-5B1-4 [High Five™]; Growth characteristics: adapted for suspension and serum-free growth, can grow efficiently at 21 or 27oC in adherent or suspension conditions, population doubling time = 21 hours, maximum growth 5 x 10e6 cells/ml. Unique properties: used for recombinant protein production with baculovirus expression systems.
Medium
Sf-900 III SFM (serum-free medium, wwwthermofishercom)
Cell Culture Collection
Available from Kerafast (https://www.kerafast.com/item/1640/trichoplusia-ni-cell-line-tni-fnl)
Status
Microorganism Propagation
Produces Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
