UCR-SE-1a
Order
Species
Common Name(s)
Beet armyworm
Primary Designation
UCR-SE-1a
Accession No.
CVCL_Z312
Stage
Tissue
Initiator(s)
W. D. Gelernter
References
Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology Progress, 7(1), 9-14; Monette, R., Savaria, D., Masson, L., Brousseau, R., & Schwartz, J. L. (1994). Calcium-activated potassium channels in the UCR-SE-la lepidopteran cell line from the beet armyworm (Spodoptera exigua). Journal of Insect Physiology, 40(4), 273-282.
Notes
Origin: clonal line of UCR-SE-1; Morphology: most cells are round and epithelial-like; Unique properties: this cell line is used to expressed recombinant proteins (pseudorabies virus glycoprotein (gp50T), human plasminogen (HPg), and β-galactosidase (β-gal)) using the baculovirus expression systems; cells possess 120-pS (in the cell-attached configuration), voltage dependent potassium channels are activated in the inside-out configuration by calcium exposure of the cytoplasmic side of the membrane (these do not appear to belong to any of the well-characterized calcium-activated potassium channels found in mammalian cells).
Medium
Grace's insect medium + 10% FBS
Status
Microorganism Propagation
Replicates Autographa californica multiple nucleopolyhedrovirus (AcMNPV)
