UFL-AG286
Order
Species
Common Name(s)
Velbetbean catepillar moth
Primary Designation
UFL-AG286
Accession No.
CVCL_Z440
Stage
Tissue
Initiator(s)
Sieburth and Maruniak
References
Sieburth, P. J., & Maruniak, J. E. (1988). Growth characteristics of a continuous cell line from the velvetbean caterpillar, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae). In vitro cellular & developmental biology, 24(3), 195-198; Hink, W. F., Thomsen, D. R., Davidson, D. J., Meyer, A. L., & Castellino, F. J. (1991). Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnology progress, 7(1), 9-14; Lynn, D. E. (2003). Comparative susceptibilities of twelve insect cell lines to infection by three baculoviruses; Chen, Y. P., Gundersen-Rindal, D. E., & Lynn, D. E. (2005). Baculovirus-based expression of an insect viral protein in 12 different insect cell lines. In Vitro Cellular & Developmental Biology-Animal, 41(1-2), 43-49; Gioria, V. V., Jäger, V., & Claus, J. D. (2006). Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line. Cytotechnology, 52(2), 113-124.
Notes
Morphology: cells are a mixture of elongated and spherical cell types; Growth characteristics: doubling time = 5.9 d when seeded at 2 X 10e5 cells/ml and 6.7 d when seeded at 3 X 10e5 cells/ml; Authentication: this cell line was characterized by isozyme analysis and from molecular weight determination of the restriction endonuclease bands of the mitochondrial DNA; Microbial presence: no mycoplasmal contaminants are present.
Medium
TC100 + 10% FBS
Cell Culture Collection
James E. Maruniak, Department of Entomology and Nematology, University of Florida, USA.
Status
Microorganism Propagation
Autographa californica NPV (AcMNPV), Anagrapha falcifera NPV (AfMNPV), and Anticarsia gemmatalis NPV (AgMNPV)
