UIV-SL-673

UIV-SL-673

Order

Common Name(s)

African cotton leafworm

Primary Designation

UIV-SL-673

Accession No.

CVCL_AV46

Stage

Tissue

Initiator(s)

D. L. Knudson

References

Knudson, D. L., Lescott T., Tinsley T.W. (1980). Establishment of a continuous cell line from Spodoptera littoralis (Lepidoptera: Noctuidae). In Vitro 16:369-370; Lynn, D (2007) Available lepidopteran cell lines. In: Methods in Molecular Biology, Vol 338: Baculovirus and Insect Cell Expression Protocols (Ed. Murhammar, D. W.), Humana Press Inc., Totowa, NJ, pp 117-138.

Notes

Morphology: this cell line are mostly epithelial-like, fibroblastlike, spherical, and spindle shaped cells; Growth characteristics: population doubling time is 30 hr; Karyology: cell line are mostly polyploid; both macro- and microchromosomes that lack distinct centromeres is observed.

Medium

TC100 + 10% FCS

Cell Culture Collection

D. L. Knudson, Unit of Invertebrate Virology, N.E.R.C., OX1 3UB, Oxford, England.

Status

Microorganism Propagation

Replicates Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), Spodoptera littoralis multiple nucleopolyhedrovirus (SlMNPV or SpliMNPV), and Trichoplusia ni single nucleopolyhedrovirus (TnSNPV)

Contact Information

S-225 Ag Science Center Lexington, KY 40546-0091

(859) 257-7450

entchair@uky.edu